Guinea pig horizontal cells express GABA, the GABA-synthesizing enzyme GAD65, and the GABA vesicular transporter
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منابع مشابه
GABA transporter-1 and GABA transporter-3 in the modulation of extracellular GABA and GABAergic tonic conductances in the rat
Tonic γ-aminobutyric acid (GABA)A receptor-mediated signalling controls neuronal network excitability in the hippocampus. Although the extracellular concentration of GABA (e[GABA]) is critical in determining tonic conductances, knowledge onhow e[GABA] is regulated by different GABA transporters (GATs) in vivo is limited. Therefore, we studied the role of GATs in the regulation of hippocampal e[...
متن کاملVigabatrin induces tonic inhibition via GABA transporter reversal without increasing vesicular GABA release.
Two forms of GABAergic inhibition coexist: fast synaptic neurotransmission and tonic activation of GABA receptors due to ambient GABA. The mechanisms regulating ambient GABA have not been well defined. Here we examined the role of the GABA transporter in the increase in ambient [GABA] induced by the anticonvulsant vigabatrin. Pretreatment of cultured rat hippocampal neurons with vigabatrin (100...
متن کاملA palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER-Golgi and post-Golgi membranes.
GAD65, the smaller isoform of the enzyme glutamic acid decarboxylase, synthesizes GABA for fine-tuning of inhibitory neurotransmission. GAD65 is synthesized as a soluble hydrophilic protein but undergoes a hydrophobic post-translational modification and becomes anchored to the cytosolic face of Golgi membranes. A second hydrophobic modification, palmitoylation of Cys30 and Cys45 in GAD65, is no...
متن کاملGABA transaminase inhibition induces spontaneous and enhances depolarization-evoked GABA efflux via reversal of the GABA transporter.
The GABA transporter can reverse with depolarization, causing nonvesicular GABA release. However, this is thought to occur only under pathological conditions. Patch-clamp recordings were made from rat hippocampal neurons in primary cell cultures. Inhibition of GABA transaminase with the anticonvulsant gamma-vinyl GABA (vigabatrin; 0.05-100 microm) resulted in a large leak current that was block...
متن کاملGABA receptors on horizontal cells in the goldfish retina
We investigated the localization of GABA(A) and GABA(C) receptors in horizontal cells (HCs) and HC axon terminals (ATs) dissociated from goldfish retina, using whole-cell patch-clamping recordings. Applications of GABA on HCs induced two groups with inward currents at the holding potential of -50 mV: One was a sustained inward current in the H1 cell, with one type of HCAT (AT1), and the other w...
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ژورنال
عنوان ژورنال: The Journal of Comparative Neurology
سال: 2010
ISSN: 0021-9967,1096-9861
DOI: 10.1002/cne.22294